Importance of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase tyrosine 707 residue for chair-boat bicyclic ring formation and deprotonation reactions.

نویسندگان

  • Tung-Kung Wu
  • Tsai-Ting Wang
  • Cheng-Hsiang Chang
  • Yuan-Ting Liu
  • Wen-Shiang Shie
چکیده

A contact mapping strategy was applied to identify putative amino acid residues that influence the oxidosqualene-lanosterol B-ring cyclization reaction. A bicyclic intermediate with two altered deprotonation products, in conjunction with lanosterol, were isolated from the ERG7(Y707X) mutants, indicating that the Tyr707 residue may play a functional role in stabilizing the chair-boat bicyclic C-8 cation and the lanosteryl C-8/C-9 cation intermediates.

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عنوان ژورنال:
  • Organic letters

دوره 10 21  شماره 

صفحات  -

تاریخ انتشار 2008